中国生物工程杂志

Abstract: Objective: To screen gravity related genes of silkworm Embryogenesis. Methods: Suppression subtractive hybridization (SSH) was used to isolate gravity related genes of silkworm embryogenesis in simulated weightlessness and normal gravity environment. Differential expression genes were cloned, sequenced and analyzed on homology. Results: 34 gravity related genes were obtained using SSH. 16 genes were up regulated in simulated weightlessness environment, including 15 unknown genes and one known gene maintaining the stability of mRNA. 18 genes were down regulated in simulated weightlessness environment, including 3 unknown genes, 4 contig genes, 5 submitted and unknown function genes in SilkBase, and 6 protein synthesis genes. Conclusion: Simulated weightlessness environment affects the expression of genes playing a role in maintaining the stability of mRNA and synthesizing protein during the period of silkworm embryogenesis.