中国生物工程杂志

In this paper, the type of basic media and the contents of plant growth substances were investigated by orthogonal design experiment, and also the effects of different culture conditions on the growth of suspension cells and the accumulation of total flavonoids in Eucommia ulmoides were studied. The results showed that B5 medium supplemented with 0.5mg/L NAA, 0.6mg/L 6-BA and 30g/L sucrose, at initial pH 5.0-5.5, 20g (FW)/L inoculation quantity and 110 rpm of rotation speed was a preferable culture conditions for E. ulmoides suspension cells growth and flavonoids synthesis. The results of metabolic kinetics analysis for E. ulmoides cell suspension culture showed that the logistic and Luedeking-Piret equations can be used for describing the kinetics of cell growth, sucrose consumption and flavonoids production during the process. The maximum specific growth rate ( m), the actual growth yield based on sucrose (YG) and maintenance coefficient (m) were 0.417d-1, 0.619g/g and 0.0206g/(g·d-1) respectively. All these outcomes in this paper could give a basis for establishing the suspension cell culture of E. ulmoides and production of the natural active components in large-scale.