TTI gene coding for Tsetse thrombin inhibitor was modified with E.coli bias codon and expressed in Escherichia coli with high efficiency. Recombinant protein was purified to more than 98% purity. Assay for enzyme activity determination was set up. The result showed that the fusion protein exhibited inhibiting activity for thrombin. Inhibitory rate of purified TTI was 73% when concentration of thrombin and substrate was 10U/ml and 250 mol/L respectively. Inhibition pattern was determined as competitive with ki at 35 mol/L.
Received: 30 April 2008
Published: 25 October 2008
