中国生物工程杂志

Abstract: RNA interference (RNAi) is a phenomenon of special post-transcriptional gene silencing mediated by induced dsRNA with endogenous or exogenous homogenous sequences, a lot of researches have been applied to demonstrate it as a reserved mechanism for gene regulation in animals, plants and fungi. In the past decade, RNAi aimed at composition of crop for nutrition improvement had been developed and tested, which included improving the quality of fat and oils, reforming starch quality, improving crop quality through altering composition of crop, elevating anti-browning nature, prolonging fruit-store, procuring secondary metabolite by metabolism regulation. This technology, as a knockdown technology, has been applied to a wide range of species to research plant gene function,silence the expression of specific genes for crop genetics breeding and offers alternative approaches for improving crop quality. The progress and potential of these approaches and studies mentioned above were reviewed and the problem about RNAi technology also was preliminarily discussed in this paper.