中国生物工程杂志

Honggumycin, which showed a significant antibacterial activity, was a bioactive compound obtained by the fermentation broth of Streptomyces 702. Under the shaking-flask culture, unsterile fermentation process of honggumycin produced by Streptomyces 702 were studied by single-factor experiment design. The optimum unsterile shaking-flask cultivation condition consisted of: 7.5% of inoculation size (v/v), 1.5mL of antimicrobial agent addition. Under the optimal conditions, honggumycin yield of unsterile fermentation was up to 1.46g/L, which was increased by 25.7% than that of sterile fermentation. The result showed that fermentation for Streptomyces 702 under non-sterile conditions was an potentially efficient fermentation mode, which not only saved fermentation cost, but also increased fermentation productivity.