中国生物工程杂志

A method to determine dihydroxyacetone (DHA) in fermentation broth was developed by high performance liquid chromatography (HPLC). DHA was separated on a Alltima C18（5μm，250×4.6mm）. The mobile phase was 0.5% methanol solution (pH adjusted to 3.0 with H3PO4), the flow-rate was 1.0 mL/min and the detective wavelength was 200 nm. The detection limits of DHA was 0.1 g/L～10.0 g/L. 6.2 g/L DHA in the fermentation broth was detected by HPLC method, which was in agreement with the result by spectrophotometric method. The method in this paper was simple, rapid and applicable for DHA determination in the fermentation broth. The method was applicable for DHA determination in the fermentation process.