中国生物工程杂志

Insects exhibit a particular resistance to infections. The activation of the innate immune response of insects is involved in the recognition of the infectious non-self and subsequent activation of cellular and humoral reactions. In humoral reactions, insect anti-bacterial peptides and lysozyme are very important in resistance to infections. Housefly (Musca domestica) is one of the most important kinds of insects and it has strong ability to adapt to the adverse circumstances. It is of momentous theoretical and practical significance to research the immunity system of housefly. In this research, the methods of inducement of housefly larvae were firstly studied. Then an antibacterial protein, whose molecular weight is 28kDa, was purified from housefly larvae, induced by 30% H2O2, through salt-out, Sephadex G-25 column, Sephadex G-75 column and CM-Sepharose Fast Flow column. This antibacterial peptide had activities against most of Gram positive and Gram negative bacteria.