中国生物工程杂志

To identify specific protein markers for gastric cancer detection and diagnosis, as well as develop new potential therapeutic targets of the disease. Gastric cancer tissues and adjacent normal mucosa were examined by the fluorescence differential in-gel electrophoresis (DIGE) technique after labeled with CyDye DIGE fluors Cy3, Cy5 and Cy2. Protein spots detected differential with statistical significance were identified by MALDI-TOF MS or MS/MS. As the result, intensity changes of 33 spots were detected with statistical significance. 9 protein spots of them up-regulated otherwise 24 down-regulated in gastric cancer tissues. And 22 of them were identified by MALDI-TOF MS or MS/MS successfully. Several proteins up-regulated such as MnSOD, HSP60, mutant desmin et al. HSP27, prostaglandin F synthase, SeBP 1, zinc finger protein 160, tubulin alpha 6, eTEF1 A 1 and so on were down-regulated. Our results suggest that DIGE is a useful technique for differential expressed proteins screening and analysis in gastric cancer tissues which may be useful for the development of new molecular markers for diagnosis and prognosis of gastric carcinoma. This differently expressed proteins may be useful tumor markers for gastric cancer.