Abstract Microsystems give new platforms for cell research. Cell patterning technology, which is an absolutely new way of cell culturing, plays an important role in the field of “cells on chip”. Here, we reviewed the main strategies of cell patterning, including photolithography, soft lithography, stencil-assisted and so on. Furthermore, we introduced the main uses of the microsystems with cell patterning technology, such as fundamental studies in cell biology, tissue engineering and cell-based biosensors.
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