Cloning the multi-gene of isoflavonoid biosynthetic pathway with the SMART strategy

China Biotechnology

2007, Vol. 27

Issue (6): 66-70 DOI:

Cloning the multi-gene of isoflavonoid biosynthetic pathway with the SMART strategy

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Abstract

High quality of total RNA from soybean leaves suitable for high efficient construction of cDNA library and gene cloning based on SMART strategy was isolated by the improved Acidic-Phenolic method. Five full length genes of isoflavonoid biosynthetic pathway were successfully and quickly amplified by PCR method with the original library cDNA dilution as templates. Comparing with traditional method of cloning genes from DNA or RNA, or isolating genes from cDNA plasmid hosted bacteria, this method can obtain full-length multi-gene more quickly and simply.

Key words： SMART cDNA strategy cDNA library Gene cloning Isoflavonoid biosynthetic pathway

Received: 09 February 2007 Published: 25 June 2007

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Cite this article:

. Cloning the multi-gene of isoflavonoid biosynthetic pathway with the SMART strategy. China Biotechnology, 2007, 27(6): 66-70.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2007/V27/I6/66

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