Micro-well protein array technology applied in monoclonal antibody isotyping

China Biotechnology

2007, Vol. 27

Issue (6): 56-60 DOI:

Micro-well protein array technology applied in monoclonal antibody isotyping

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Abstract

Monoclonal antibody isotyping micro–well protein array was designed and established. Twelve monoclonal antibodies and two polyclonal antibodies were isotyped by the isotyping protein array and ELISA respectively. The results showed that there was no difference between protein array method and ELISA in the application of isotyping monoclonal and polyclonal antibodies. And fewer sample and materials were used and the efficiency was improved in protein array method compared with ELISA. As a result, the antibody isotyping protein array could be used as a sensitive, rapid, directional and economical tool applying in high throughput monoclonal antibody generation.

Key words： protein array monoclonal antibody isotype

Received: 01 February 2007 Published: 25 June 2007

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Cite this article:

. Micro-well protein array technology applied in monoclonal antibody isotyping. China Biotechnology, 2007, 27(6): 56-60.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2007/V27/I6/56

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