Transgenic tobacco expressing the crocus CsZCD gene showed the production of crocetin

China Biotechnology

2007, Vol. 27

Issue (6): 51-55 DOI:

Transgenic tobacco expressing the crocus CsZCD gene showed the production of crocetin

Download:

HTML

PDF(559KB) HTML
Export: BibTeX | EndNote (RIS)

Abstract

To investigatethe feasibility to produce crocetin in tobacco plants. In this study, the coding region of zeaxanthin cleavage dioxygenase (CsZCD) gene from crocus sativus L. was inserted into the downstream of the cauliflower mosaic virus (CaMV) 35S promoter of a binary vector pBI121, and integrated into the genome of Nicotiana tabacum L. Twenty-one transgenic lines were identified by genomic southern blotting analysis. Western blotting and HPLC analysis of the leaf extracts of transgenic tobacco showed that crocetin was produced in CsZCD gene-transformed plants, while no crocetin was found in the untransformed tobacco.

Key words： Saffron Crocetin Crocin Zeaxanthin cleavage dioxygenase Transgenic tobacco

Received: 01 February 2007 Published: 25 June 2007

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors

Cite this article:

. Transgenic tobacco expressing the crocus CsZCD gene showed the production of crocetin. China Biotechnology, 2007, 27(6): 51-55.

URL:

https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2007/V27/I6/51

[1]	Min YAO,Shu-hua ZHU,Fo-sheng LI,Shi-yan ZHANG,Lin TANG. *Analysis of Salt Tolerance and Insect Resistance of Transgenic Tobacco Expressing AtCYSa* from Arabidopsis**[J]. China Biotechnology, 2018, 38(4): 8-16.

[2]	HAN Shuang, YANG Zhi-li, CHEN Li-mei. *Over Expression of Arabidopsis CAT* Improved the Absorption and Tolerance of Gas HCHO in Tobacco**[J]. China Biotechnology, 2015, 35(5): 41-48.

[3]	LIU Lei, SUN Zhen, SONG Zhong-bang, XIAO Su-qin, CHEN Li-mei. Simultaneous Over-expressions of AOD1 and HPS-PSI in Chloroplasts Creates a Novel Photosynthetic CH₃OH-assimilation Pathway and Enhances Its Ability to Assimilate CH₃OH[J]. China Biotechnology, 2013, 33(12): 69-78.

[4]	SONG Feng, SUN Min, LUO Ke-Meng. A Simple and Rapid PCR-based Method for Identifying Transgenic Tobacco Plants Carrying a Single Copy of the Integrated Gene[J]. China Biotechnology, 2010, 30(04): 83-88.

[5]	HU Li-E, SONG Wei, LIU Xiao-Hui, JIANG Ting, DIAO Ling-Xia. Expression of Bioactive Thymosin α1 Using Marker Free Transgenic Tobacco[J]. China Biotechnology, 2010, 30(03): 1-8.

[6]	. Expression of a kind of curcin induced from Jatropha curcas (curicin 2) in tobacco[J]. China Biotechnology, 2007, 27(4): 94-98.

[7]	. Induction and screening of Gardendia jsminoide callus lines for crocin production[J]. China Biotechnology, 2006, 26(06): 50-54.

Viewed

Full text

Abstract

Cited

Shared

Discussed