The Study of Isolation , Purification and Growth Behavior of Bovine Sertoli Cells

China Biotechnology

2007, Vol. 27

Issue (5): 96-100 DOI:

The Study of Isolation , Purification and Growth Behavior of Bovine Sertoli Cells

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Abstract

In order to study the growth behavior of bovine Sertoli cells, adopting the combination of enzymatic digestion and different attaching velocities methods to isolate and purify Sertoli cells of 5-month, 6-month bovine fetal testis and newborn bovine testis, then cultured in vitro. Indicating that bovine testis in this stage were suitable for isolating and purifying Sertoli cells, 0.25% trypsin+0.02%EDTA is an economic and efficient method to isolate bovine fetal Sertoli cells, and the optimal research period of bovine fetal Sertoli cells should be controlled within 3-20 days, bovine fetal Sertoli cells in exponential growth stage can obviously promote the development of bovine IVF zygotes in vitro.

Key words： bovine Sertoli cells in vitro culture growth behavior

Received: 25 January 2007 Published: 25 May 2007

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Cite this article:

. The Study of Isolation , Purification and Growth Behavior of Bovine Sertoli Cells. China Biotechnology, 2007, 27(5): 96-100.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2007/V27/I5/96

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