The prokaryotic expression of Porcine Resistin and its purification, identification

China Biotechnology

2007, Vol. 27

Issue (5): 16-20 DOI:

The prokaryotic expression of Porcine Resistin and its purification, identification

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Abstract

Resistin is a newly discovered adipocyte hormone.With the method of gene engineering,we got resistin.The RSTN gene was subcloned to the expression vector pET-32a(+) and the recombined plasmid named pET-RSTN was constructed successfully. The plasmid pET-RSTN were transformed into the Eoli.BL-21(DE3). After inducing by IPTG the fusion protein of resistin were expressed and its molecular weight was about 30kDa. As temperature、IPTG concentration and induction time were optimized,the soluble recombinant protein was high expressed at 30℃ for 4h with 1mmol/L IPTG induction. The product was identified by SDS-PAGE and western-blotting. The expression production purified on Ni2+-NTA column.

Key words： resistin protein expression purification

Received: 31 October 2006 Published: 25 May 2007

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Cite this article:

. The prokaryotic expression of Porcine Resistin and its purification, identification. China Biotechnology, 2007, 27(5): 16-20.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2007/V27/I5/16

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