The Development of Rapid PCR

China Biotechnology

2007, Vol. 27

Issue (4): 99-103 DOI:

The Development of Rapid PCR

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Abstract

PCR is one of the common techniques in molecular biology, which can amplify nucleic acids through the cycle of denaturation, annealing and extension. Based on the principle of common PCR, rapid PCR is to realize the amplification of nucleic acids in less time without affecting the specificity, sensitivity and fidelity of the reaction. A lot of research work in this field has been going on in recent years. And this article will make a review of the development of rapid PCR with emphases on the improvement of DNA polymerase, the choice of additives and the improvement of thermocyclers.

Key words： rapid PCR DNA polymerase fusion protein thermocycler

Received: 12 October 2006 Published: 25 April 2007

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Cite this article:

. The Development of Rapid PCR. China Biotechnology, 2007, 27(4): 99-103.

URL:

https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2007/V27/I4/99

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