中国生物工程杂志

The spermatozoa from mature guinea pigs were incubated in modified TALP under 5% CO2 in air at 37 ℃. The capacitation condition was assessed by chlortetracycline (CTC) staining, the localization and level of tyrosine phosphoryalted proteins during the capacitation were detected by indirect immunofluorescence or Western blot. The results showed that the number of sperm tyrosine protein phosphorylated increased from 31% to 92% during capacitation period. The site of phosphorylated tyrosine-specific fluorescence was expanded from the head of spermatozoa to the whole head and principal and mid-piece areas of the flagellum. 40kDa protein in sperm extracts taken at 0 to 0.5h after incubation was detected by anti-phosphotyrosine monoclonal antibody. 1h after incubation, another protein of 80kDa was found and the level of this protein reached the highest point at 3h. 3h after incubation, three proteins with relative molecular weight of 40, 45, 80kDa were detected.