Application of a Two-stage Temperature Control Strategy for CF66I in Batch Fermentation By Burkholderia cepacia CF-66

China Biotechnology

2007, Vol. 27

Issue (4): 66-70 DOI:

Application of a Two-stage Temperature Control Strategy for CF66I in Batch Fermentation By Burkholderia cepacia CF-66

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Abstract

Batch fermentation of antibiotic substance CF66I by Burkholderia cepacia CF-66 at various temperatures ranging between 23℃～33℃ were investigated and the dynamics characteristics during fermentation process were also analyzed. Based on the results, a two-stage temperature-shift strategy in which temperature at 30℃ was controlled at first 20 h, and then changed to 25℃ till the end of fermentation was developed. By applying this temperature-shift strategy in CF66I fermentation, the CF66I activity reached 3.783 u/ml and increased 26.1%, compared to single temperature at 23℃, 25℃, 28℃, 30℃ and 33℃.

Key words： biological pesticide Burkholderia cepacia CF-66 CF66I batch fermentation two-stage temperature-shift strategy

Received: 04 September 2006 Published: 25 April 2007

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Cite this article:

. Application of a Two-stage Temperature Control Strategy for CF66I in Batch Fermentation By Burkholderia cepacia CF-66. China Biotechnology, 2007, 27(4): 66-70.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2007/V27/I4/66

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