Purification of Kallikrein Using Reverse Micellar Extraction

China Biotechnology

技术与方法

Purification of Kallikrein Using Reverse Micellar Extraction

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Abstract The purification of Kallikrein using CTAB/hexanol/octane reverse micelles extraction has been studied and optimized，under various aqueous pH values, ionic strength and species, CTAB concentration and co-surfactant concentration. The result shows that the activity recovery is more than 80%, the commercial enzyme activity is increased by 1.97 times and the crude enzyme activity is increased by 7.15 times under the conditions of［CTAB］＝0.02 mol•L-1, hexanol/octane (V/V)＝1：5, pH=9.0 and［KBr］＝0.1 mol•L-1 in forward extraction, pH＝7.0 ,［KBr］＝1.5 mol•L-1 and 15% ethanol(V/V) in backward extraction. The result of purified Kallikrein is examined by the SDS-PAGE analysis. Reverse micelles extraction is a potential technique for the application in the downstream biotechnological processes.

Key words： CTAB Kallikrein Reverse micelles extraction

Received: 23 October 2006 Published: 25 March 2007

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. Purification of Kallikrein Using Reverse Micellar Extraction. China Biotechnology, 2007, 27(3): 93-99.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2007/V27/I3/93

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