中国生物工程杂志

Mitochondrion is the basic and important organelle in most of the eukaryocyte, It is the place of oxidative phosphorylation. The gene order of the different vertebrate shows that the gene of cytochrome b(Cytb)、cytochrome oxidase I（COⅠ）、cytochrome oxidase II（COⅡ）and cytochrome oxidase III（COⅢ）are the most consevative,The homology is the highest. The genovariation of ATPase6、ATPase8 and ND is more much.The mitochondrial cytochrome oxidase III(COⅢ) and its associated tRNA-Gly、ATPase6 genes of domestic duck were sequenced by PCR.The open reading frame of COⅢ gene contains 784bp nucleotides,The COⅢ gene of domestic duck shows a high degree of homology with that from the other poultry recorded in the GenBank and it has 90.6% homology with Aythya Americana. 89% with Branta canadensis、88.6% with Anser albifrons and 88.6% with Cygnus columbianus.The phylogenetic trees show that the relationships based on the homology is consistent with the morphological and taxonomic results.