Construction of an Inducible Expression and Secretion System for Neutral Protease in Bacillus subtilis

China Biotechnology

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Construction of an Inducible Expression and Secretion System for Neutral Protease in Bacillus subtilis

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Abstract The promoter and signal peptide sequence of sacB gene (sacR gene) has been amplified by PCR．An inducible expression and secretion vector pHP13SN has been constructed with this amplified sequence，which was ligated with the pro-peptide and mature peptide of neutral protease gene on the vector pHP13．Transforming Bacillus subtilis DB104 with the vector pHP13SN, and the recombinant strain DB104(pHP13SN) can be got．The neutral protease gene has been expressed by the inducement of sucrose and the regulation of sacR，and the production has been secreted with bioactivity．

Key words： Inducible expression Bacillus subtilis Neutral protease

Received: 23 November 2006 Published: 25 March 2007

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. Construction of an Inducible Expression and Secretion System for Neutral Protease in Bacillus subtilis. China Biotechnology, 2007, 27(3): 105-109.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2007/V27/I3/105

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