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Screening of γ-Polyglutamic Acid High Productive Strain Using Crop Materials and Optimization of Fermentation Condition |
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Abstract One strain Bacillus subtilist B-115 was screened, which can use crop materials to product γ-Polyglutamic acid. The high productive strain B-115 of γ-PGA was obtained from the primitive strain Bacillus subtilis B-1through UV mutagenesis. The yield of γ-Polyglutamic acid increased from original 12.5g/L to 19.5g/L by flask fermentation. In order to enhance the production of this strain, we used Response Surface Methodology (RSM) to optimize fermentation medium ,such as carbon source, nitrogen source, concentration of sodium glutamate, metal ions, the final yield of γ-PGA was upto 40.98g/L.
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Received: 27 September 2007
Published: 25 December 2007
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