High-Level Expression of Penicillinase Gene in Bacillus Subtilis

China Biotechnology

2007, Vol. 27

Issue (12): 31-35 DOI:

High-Level Expression of Penicillinase Gene in Bacillus Subtilis

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Abstract

To obtain a number of extracellular penicillinase, the gene (penp) encoding penicillinse of Bacillus cereus ATCC10987 was cloned into expression vector in Bacillus subtilis , transformed into Bacillus subtilis DB104 deficient in tow proteases。When recombinant bacteria was cultured in LB medium for 24 hours, the result of SDS-PAGE showed that the molecular weight of the protein was 28KDa and the penicillinase activity reached 339U/ml; By screening severn different fermentation media, the result showed that 4# medium is favorable to producing penicillinase by the recombinant cells than the others, with the yield of the enzyme being 1580U/mL; when the recombinant cells was cultured in 7 liter fermentor for 24h ,the penicillinase activity reached 1255.8U/ml．

Key words： Penicillinase Heterologous Expression Bacillus subtilis

Received: 18 September 2007 Published: 25 December 2007

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. High-Level Expression of Penicillinase Gene in Bacillus Subtilis. China Biotechnology, 2007, 27(12): 31-35.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2007/V27/I12/31

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