中国生物工程杂志

Six hemicellulose-decomposing strains were isolated from the refuse soil samples using bagasse hemicellulose as the sole carbon. The strain with high xylanase activity was screened and identified as Aspergillus sp. HQ3 by analysis of 18S rDNA sequences (97% similarity to Aspergillus sp.) and phenotypic properties. The optimum solid-state fermentation conditions for xylanase production were obtained as: bagasse: bran =7: 3 (W/W), solid: liquid =1: 4 (W/W), urea 0.4%, pH 7.0, fermentation temperature 30 ℃, fermentation time 4d. The activity of xylanase reached 3421 U/g koji under the optimum conditions.