中国生物工程杂志

To study hepatic stem cells(HSCs) proliferation and characteristic in rotary cell culture system(RCCS) for ideal cell origin of liver tissue engineering and clinic liver diseases stem cells therapy. WB-F344, a kind of rat liver stem cell, was cultured in RCCS to investigate the effect of RCCS on hepatic stem cell characteristics, and the condition of two-dimensional (2D) monolayer cultivating as control. To analyze the changing of HSCs characteristics by morphological observation, flow cytometry（FACS）, RT-PCR and immunofluorescence. To the results, demonstrated that cells were grow well and packed together densely to form irregular aggregates in RCCS, and proliferation of the hepatic stem cells was superior with control, mRNA of AFP increased while ALB decreased, the protein of AFP expression increased while ALB decreased comparing to control. Indicating RCCS could afford appropriate environment to sustain the stem cells character of WB-F344 cells.