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| Preparation and Characteristic of Monoclonal Antibodies to tag of FLAG and Its Primary Application |
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Abstract Monoclonal Antibodies against FLAG, which was conjugated with BSA by EDC, were produced using conventional cell fusion technology.Five high affinity hybridoma lines were screened for specificity to FLAG by ELISA ，their ascites titers were higher than 1：106.Those monoclonal antibodies didn’t have cross reactivity with other antibiotics, and were implied in Immuno-affinity Chromatography successfully .It may be applicated in purification and research of syncretic protein with tag.
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Received: 09 August 2006
Published: 10 May 2010
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