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| Effect of culture conditons on Jarosite for immobilized Acidothiobacillus ferrooxidans |
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Abstract With Ca(NO3)2 (1%~5%) solution as crosslinking agent, Acidithiobacillus ferrooxidans was immobilized by the complex of PVA solution and sodium alginate solution. Continuous culture of immobilized Acidithiobacillus ferrooxidans can be used to remove H2S and SO2. In order to reduce the negative effect of jarosite which formed during the oxidation of Fe2+ by immobilized cells, the changing of initial pH and the ingredient in 9K medium were tried. The results showed: among the three methods, decreasing the concentration (NH4)2SO4 was more feasible than two others. By decreasing the concentration of (NH4)2SO4 from 3 g/L to 0.5 g/L in batch culture, the forming rate of jarosite was decreased by 50%, while Fe2+ oxidation rate was hardly affected. Based on the results from batch culture, a modified 9K medium containing less ammonium sulfate was used to develope an immobilized bioreactor system in which long-term continuous ferrous iron oxidation for about 96h of operation at dilution rates of 0.4 h-1, were successfully achieved with a small amount of jarosite which has many negative effects and a high Fe2+ oxidation rate of 3.75 g/L.h .
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Received: 04 September 2006
Published: 10 May 2010
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