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| Generation of the Antibody against the schistosoma japonicum antigen Sj22 with genetic immunization |
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Abstract To study the genetic immunization effectiveness, 16 6-week-old female BALB/C mice were immunized with recombinant plasmids with or without CpG adjuvant, and then boosted with the same recombinant plasmids or the recombinant protein of Schistosoma Japonicum antigen Sj22 in four experimental groups. Mice in group A were immunized with 100μg of plasmid pVAX1-sj22. Mice in group B were immunized with 50μg plasmid pVAX1-sj22 and 20μg CpG adjuvant simultaneous. Mice in group C and D were immunized with pcDNA3.1-sj22. All the mice were immunized at the 0, 3rd, and 6th week by i.m. injection and boosted with recombinant Sj22 protein except group D boosted with the plasmid pcDNA3.1-sj22 at the 9th week. Blood was collected at the 0, 9th and 10th week, and the titer of the serum was detected by ELISA, the specificity of the antibody was examined by Western blot. In conclusion, the antiserum specified to Sj22 protein was obtained by genetic immunization. And the study shows that the application of CpG adjuvant can reduce the amount of the plasmid and the DNA prime-boost protein strategy can improve the effectiveness of immunization reaction in genetic immunization.
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Received: 11 September 2006
Published: 10 May 2010
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