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| Disruption of hom gene encoding for homoserine dehydrogenase of Corynebacterium glutamicum |
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Abstract In this research the hom gene encoding for homoserine dehydrogenase was amplified from the genomic DNA of Corynebacterium glutamicum ATCC 13032. After the kanamycin-resistant gene (Km) cassette from plasmid pET28a was inserted into the center of hom, the hom::Km cassette was then electroporated into the competent cell of C. glutamicum ATCC 13032. And kanamycin-resistant clones were obtained. PCR was performed to confirm whether the Km gene was integrated into the hom gene of these clones and the recombinant strains of hom-disrupted were screened out. Fermentation results showed that the lysine yield of the hom-disrupted strain C.g-hom::Km-8 reached 4.7 g/L, which was 6.7 times that of C. glutamicum ATCC 13032.
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Received: 26 July 2006
Published: 10 May 2010
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| Fund: the National Natural Science Foundation of China |
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