Construction of OTX1 lentiviral vector and overexpression research

China Biotechnology

2007, Vol. 27

Issue (1): 16-21 DOI:

Construction of OTX1 lentiviral vector and overexpression research

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Abstract OTX1 gene is one of the pivotal transcriptional factors involved in the neurogenesis. In order to overexpress the OTX1 gene in distinct cell types and find out its contribution to the proliferation and differentiation of stem cells in vitro, OTX1 cDNA was subcloned into lentiviral vectors. The resulting constructions pDUET－OTX1、pDUET－GFP－OTX1 and pDUET-GFP were packaged in 293 cells producing viral particles to transduce 293T cells、SY5Y cells、mouse embryonic stem cells and E15 neural stem cells. It was proved that the transferred OTX1 gene was located in the nuclei of the transduced cells in stead of plasma. Lentivirus is an ideal vector delivering gene to different cells. The overexpression of OTX1 in transduced 293T cells were validated by Western blot and immunofluorescence.

Key words： lentivirus OTX transcriptional factor gene expression

Received: 11 September 2006 Published: 10 May 2010

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	REN Ping
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. Construction of OTX1 lentiviral vector and overexpression research. China Biotechnology, 2007, 27(1): 16-21.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2007/V27/I1/16

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