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| Activity study of ciliary neurotrophic factor (CNTF)mutant |
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Abstract To study furderly the activity of CNTF mutant designed by computer molecular modling,this study used the methods of dissociated cultures of chick dorsal root ganglion、TF-1 prolification and the normal mice′weight loss tests.The results indicated that the mutant protein promoted the survival of dorsal root ganglion、induced TF-1 prolification and made the normal mice lose weight,decrease appetite and reduce fat index.The weight loss effect was dependant with its administration dosage,ED50 was 150.986?g/kg/d.To TF-1,the specific activity reached 2.0×106U/mg against international reference reagent.In a word,CNTF mutant had excel bioactivity.So our study provided cluds for its development and application.
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Received: 16 August 2006
Published: 10 May 2010
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