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Abstract To find the different resistance-related proteins of magnaporthe grisea by using two-dimensional gel electrophoresis for understanding the mechanism of the resistance of rice against magnaporthe grisea. The total proteins of four kinds of callus inductions were isolated by TCA/cold acetone precipitation. The four kinds of total proteins were prepared by means of immobilized pH gradient based on two-dimensional gel electrophoresis. After silver staining, gel-image analysis was performed by using PDQuest. Using proper method stated above, satisfactory 2DGE maps of total proteins obtained and the preliminary analysis results were reported. The results showed that average numbers of resistant variety protein spots were 447 and 523 before and after Magnaporthe grisea infestation, and the corresponding average matching rate were 89% and 87%. The average numbers of susceptible variety protein spots were 440 and 326, and the corresponding average matching rate were 86% and 85%.The 76 differential protein spots were identified between the two types of resistant variety tissues, and the 114 differential protein spots were identified between the two types of susceptible variety tissues. The differentially expressed proteins can be observed in resistant and susceptible rice to Magnaporthe grisea, which can be used to elucidate the pathogenesis of Pyricularia grisea for further study.
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Received: 26 September 2006
Published: 25 December 2006
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