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Abstract The original strain was treated with UV combining LiCl. The result showed that strain UL-40 has a good stability and higher capacity to synthesize astaxanthin under 25℃. The total carotenoid yield was 7.10mg/L. HPLC analysis showed that the maximal yield of astaxanthin of the selected strain had reached 643.97μg/g, which was 249.87% higher than that of the initial strain. The fermentation temperature, initial pH of culture solution and 6 kinds of substrate were optimized by Plackett-Burman design and Response Surface Analysis provided by SAS software. The temperature, initial pH and corn steep liquor were chosen as the major factors from all the 8 variables, a regression model equation was obtained after regression analysis performed on the data from RSA experiment. The optimal conditions were predictted to be 16.78℃, pH4.73 and 7.06 mg/L, respectively, for the maximum carotenoid production. Under these conditions, the theroretical maximal yield of carotenoid was 3.9407 mg/L. The model was validated experimentally and the practical maximal yield of carotenoid was about 3.9261mg/L. The carotenoid production was increased by 20.4% when P. rhodozyma was cultivated under the optimal conditions as compared with the control.
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Received: 26 June 2006
Published: 25 December 2006
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