| 研究报告 |
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| Construction of Nucleic Acid Vaccine of Porcine Parvovirus and Research on Immunogenicity |
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Abstract VP2 gene of Porcine Parvovirus (PPV) was inserted into eukaryotic expression vector. Then the eukaryotic expression vector, PCIneo-VP2 plasmids was constructed. The plasmid was transfected to PK-15 cells. The expressed VP2 protein were detected by direct fluorescentantibody in vitro. Inoculated mice with PCIneo-VP2 by muscle injection and detected antibody of PPV by competitive ELISA. And in order to study immune effect of nucleotide vaccine, we inspected the invert function of lymphocyte and the cytotoxic activity by MTT. The results suggested that the mice inoculated with PCIneo-VP2 can produce antibodies after a week. At the fourth week, the level of antibodies grew to peak. These have a coincidence with the control groups injected with live vaccines. By lymphocyte growth test, we found that PCIneo-VP-2 and live vaccines could induce the increase of T-lymphocyte, but the increase of B-lymphocyte couldn’t be induced, and they could inhance the cell toxicity. The experiment proved that the humoral immunity and cellular immunity could be induced efficiently by PCIneo-VP2.
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Received: 07 July 2006
Published: 25 December 2006
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