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Abstract The array CGH technique (Array Comparative Genome Hybridization) has been developed to detect chromosomal copy number changes on a genome-wide and/or high-resolution scale. It is used in human genetics and oncology. Generally PCR amplified bacterial artificial chromosomes (BACs) or cDNAs have been spotted as elements on the array. Recently Oligonucleotides as spotted elements on the arrays have been successfully explored. Compared with BAC array CGH, oligonucleotide array CGH(oaCGH) can save considerable time and efforts, be designed with more flexibility and provide much higher resolution with high sensitivity . We expect a gradual transition from BAC array CGH to oligonucleotide array CGH in the coming years. The combination of oaCGH and other high-through put analysis can lead to discovery of a host of novel oncogenes , tumor suppressors as well as tumor drug resistance genes. This review compares the different platforms of oaCGH and its development in recent years.
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Received: 25 May 2006
Published: 25 October 2006
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