Abstract With a pair of primers designed according to the sequence of Infectious bursal disease virus(IBDV), the VP5 gene of IBDV was amplified from vvIBDV-Gx, IBDV-Gt, respectively. Then IBDV VP5 was cloned into expressing vector pET30a and pET28a. The recombinant plasmid was identified by restrictive digestion, PCR and sequence analysis, then named pET30a-GxVP5, pET28a-GtVP5, respectively. The recombinant plasmid was transformed into E.coli BL21(DE3) and induced by IPTG. SDS-PAGE results indicated that Gx-VP5, Gt-VP5 were about 24kDa, 23kDa, respectively. These recombinant fusion proteins mainly existed as inclusion bodies. High titer anti-VP5 serum was also prepared in BALB/c mice immunized with purified Gx-VP5 fusion protein inclusion. ELISA results showed that titer was above 1:25600 and Western blot analized that the expressed protein Gx-VP5 reacted with polyclonal antibody and 6×His monoclonal antibody. It explained that the expressed protein Gx-VP5 possess specific satisfactory immunological reaction.
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