Medium Optimization for the Novel Antifungal Material by Burkholderia cepacia CF-66

China Biotechnology

技术与方法

Medium Optimization for the Novel Antifungal Material by Burkholderia cepacia CF-66

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Abstract Statistics-based experimental design was used to investigate the effect of medium components on CF66I production by Burkholderia cepacia CF-66. A fractional factorial design augmented with center points revealed that sodium citrate and yeast extract were the most significant factors, which influenced CF66I production positively, while the other factors were not important with the levels tested. The method of steepest ascent was used to approach the proximity of optimization. This task was followed by a central composite design to determine the optional concentration of sodium citrate and yeast extract. The optimized medium allowed the activity of CF66I to be increased from 2.12 U/ml to 6.24 U/ml，about 200% higher than the original medium.

Key words： CF66I response surface methodology central composite design Burkholderia cepacia CF-66

Received: 31 March 2006 Published: 25 September 2006

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. Medium Optimization for the Novel Antifungal Material by Burkholderia cepacia CF-66. China Biotechnology, 2006, 26(09): 56-60.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I09/56

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