Development of Monoclonal Antibodies of Chicken Infectious Anemia Virus VP3 Protein

China Biotechnology

技术与方法

Development of Monoclonal Antibodies of Chicken Infectious Anemia Virus VP3 Protein

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Abstract Monoclonal antibodies (McAbs) specific to CIAV VP3 proteins were developed by fusing SP2/0 myeloma cells and spleen cells of BALB/c mice immunized with the recombint VP3 protein purified by Ni-NTA colomn. Three hybridoma cell lines were examinated by indirect ELISA and cloned for three times by limited dilution. Three of them were named as AG2, BC11 and FG1 which ELISA titers of supernatant culture and ascites were 2.5ⅹ102, 1.5ⅹ102, 1ⅹ10 and 2ⅹ106, 1ⅹ106, 2.5ⅹ105 respectively. The numbers of chromosome of all McAbs are between 97 and 104. In this study, the McAbs against VP3 proteins of CIAV did not show cross-reacting to avian viruses (NDV、MDV、IBDV、REOV) and cells of MSB1 and Sf9 by ELISA indicating the specify of these McAbs and can be used to study the structure and function of VP3 protein.

Key words： indirect ELISA McAb Chicken infectious anemia virus(CIAV)

Received: 29 March 2006 Published: 25 September 2006

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. Development of Monoclonal Antibodies of Chicken Infectious Anemia Virus VP3 Protein. China Biotechnology, 2006, 26(09): 51-55.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I09/51

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