研究报告 |
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Expression of Spodoptera litura Multicapsid Nucleopolyhedrovirus VP39-GST Fusion Protein in Escherichia.coli |
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Abstract The ORF of Spodoptera litura multicapsid nucleopolyhedrovirus(SpltMNPV)vp39 gene was obtained by PCR method from SpltMNPV genomic DNA. The PCR product was cloned into pMD18-T vector to get the recombinant plasmid(pMD18-vp39).The vp39 gene was recombined in vitro with prokaryotic expression vector pGEX-4T-1 and transformed into Escherichia coli BL21(DE3). The BL21(DE3) strain, containing vp39 recombinant plasmid, expressed a 60 kD fusion protein after the induction with 1 mmol/L IPTG (Isoprophylthio-β-D-galactoside). The expression of the VP39-GST fusion protein was suitable for further analysis of the inter-action between baculovirus and its host.
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Received: 31 March 2006
Published: 25 September 2006
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