| 研究报告 |
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| Eukaryotic expression of βig-h3 gene and its effects on secretion of MMPs in the human hepatoma 7721 cells |
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Abstract βig-h3 was first identified as a transforming growth factor-beta1-inducible gene in human lung adenocarcinoma cell line. It encodes for a secreted extracellular matrix (ECM) protein, which is thought to act on cell attachment and ECM composition. Our previous study showed that Objective: To construct eukaryotic recombinant expression plasmid βig-h3/ pEGFP-C2 and βig-h3 highly expressed in human hepatoma cell lines such as human FHCC-98 with high metastatic potentials but several others such as human 7721 hepatoma cells. The present study aimed to transfect βig-h3 into 7721 cells to investigate its effect on secretion of MMPs in the transfected human hepatoma cells. Full-length βig-h3 gene，cloned by reverse transcription polymerase chain reaction (RT-PCR) was inserted into the eukaryotic expression vector pEGFP-C2. The recombinant plasmid was transfected into 7721 cells with Lipofectamine2000 and Gelatin-Zymography were adopted to detect the production of MMPs in the transfected cells. Results showed that βig-h3/pEGFP-C2 recombinant expression plasmid was successfully constructed and achieved high transfection efficiency. MMPs expression of the transfected cells was promoted significantly. These results suggest that overexpression of βig-h3 promoted the production of MMPs, indicating that βig-h3 may play roles in the invasive and metastatic processes of hepatoma.
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Received: 19 May 2006
Published: 25 September 2006
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