| 研究报告 |
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| Studies of Escherichia coli accumulating lycopene and its culturing conditions |
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Abstract Three genes crtE, crtB, crtI related to lycopene synthesis in Erwinia uredovora were cloned into pET-15b to construct expressing vector pET-15bEIB. The expressing vector was introduced into E.coli BL21(DE3) to construct engineering bacteria. The engineering bacteria could accumulate a red pigment when induced by IPTG. The red pigment was lycopene as judged by absorbance spectra analysis and HPLC analysis. The engineering bacteria could grow to 3.45gDW/L and accumulate lycopene to 5.8mg/gDW under culturing conditions. The culturing conditions used were as follows: the medium was modified LB broth（Trypton 10g/L,Yeast Extract 5g/L,Maltose 5g/L,MgSO4 0.1g/L，NaCl 10g/L）, the engineering bacteia were first shake-cultured at 37℃ to OD600 of 0.6, and then IPTG was added to a final concentration of 0.5mmol/L, the engineering bacteria was further cultured for 14h at 30℃.
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Received: 20 January 2006
Published: 25 August 2006
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