Heterologous expression of Zygosacharomyces rouxii ZrGpd 1 in Pichia farinosa

China Biotechnology

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Heterologous expression of Zygosacharomyces rouxii ZrGpd 1 in Pichia farinosa

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Abstract To examine the effects of heterologous expression of ZrGPD1 (encoding glycerol 3-phosphate dehydrogenase ) cloned from osmotolerant yeast Zygosacharomyces rouxii on glycerol production in wild Pichia farinosa, the URA3 gene was amplified from P. farinosa as the homology integrative region. A recombinant plasmid (pUR-ZG) was constructed then transformed into P. farinosa by electroporation. The transformant pfa-gu was obtained by the selectable marker ZeocinTM .Primary results showed that the biomass of pfa-gu was higher than the wild type in the flask and after 72h fermentation the concentration of glycerol of pfa-gu was 37g/L enhanced 30% in comparison with the wild type. It is concluded that heterologous expression of ZrGPD1 is useful for increasing glycerol production and the ability of osmoregulation in P. farinosa.

Key words： osmotolerant yeast Zygosaccharomyces rouxii integrative expression Glycerol Glycerol 3-phosphate dehydrogenase

Received: 31 March 2006 Published: 25 August 2006

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. Heterologous expression of Zygosacharomyces rouxii ZrGpd 1 in Pichia farinosa. China Biotechnology, 2006, 26(08): 32-36.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I08/32

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