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Abstract Recent research has proved that interferons play very important role in early embryo development. We first cloned the full length cDNA (AJ584672) of rabbit early embryo developmental new gene IFRG (interferon responsive gene). According to rabbit IFRG cDNA, intact open reading frame (full length was 396bp) was amplified using rabbit ovary cDNA as template. The purified rabbit IFRG cDNA was inserted into pGEX-4T-2 vector to construct the prokaryotic expression vector. IFRG protein was expressed in E. coli in the form of glutathione-S-transferase (GST) fusion protein. The recombinant expression plasmid was transformed into BL21 and gene expression was induced by IPTG. Expression protein were detected by SDS-PAGE and confirmed by Western blotting analysis, which showed the molecular weight of the protein was a 41kDa and had immunity activity.
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Received: 22 March 2006
Published: 25 June 2006
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