Senescence and Telomere Shorten of Goat Gene-Transfected Cells

China Biotechnology

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Senescence and Telomere Shorten of Goat Gene-Transfected Cells

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Abstract Goat fetal fibroblasts were senescent during gene transfection by either the way of electoporation or LipefectaminTM?2000 with linear pGBC4tPA. Senescent cells became greatness, and one nucleolus split to multi-nucleolus and cytoplasm was hollow. The telomeres of senescent goat fetal fibroblasts were analyzed, and the results showed that the telomere lengths of senescent cells were shorter than their primary cells significantly (8.31kb vs 10.87kb). The gene-transfected somatic cells could support the in vitro development of cloned embryos that the senescent cells as donor cells to nuclear transfer.

Key words： Telomere Gene transfection Nuclear transfer Cellular senescence

Received: 29 December 2005 Published: 25 June 2006

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. Senescence and Telomere Shorten of Goat Gene-Transfected Cells. China Biotechnology, 2006, 26(06): 45-49.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I06/45

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