| 研究报告 |
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| Studies of function and cellular distribution of PS1 using PS1/GFP fusing protein |
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Abstract Mutations in presenilin1 (PS1) gene are genetically linked to the early onset of familial Alzheimer's disease (EOFAD). We construct the fusion gene GFP-PS1 (recombinant plasmid pEGFP-C1-PS1) and PS1-GFP (recombinant plasmid pEGFP-N2-PS1) to study the cellular distribution of PS1 holoprotein. Recombinant plasmids were transiently transfected into HEK293 cell and CHO cell, and watched with SPOTⅡ（Olympus，BH2） and CONFOCAL microscope （Olympus， FV300） under 488nm. We found that PS1 locates on nuclear envelope, fewer on cellular membrane, and inhomogeneous distribution in cytosol.
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Received: 12 September 2005
Published: 25 June 2006
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