Selection of bFGF mimic peptide by phage display

China Biotechnology

研究报告

Selection of bFGF mimic peptide by phage display

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Abstract Objective: To obtain the bFGF mimic peptide binding to FGFR via phage display, and to provide the base for developing peptide agonist of bFGF. Methods: Using Balb/c 3T3 cells as the target cells and COS-7 cells as the subtractive panning, the phage display heptapeptide library was biopanned for 4 rounds to obtain the single phage clones. The affinity and the specificity of the clones were assessed by ELISA.DNA sequencing was applied to further analyze the positive clones. Results: Twelve positive clones were selected from the enriched phages. A group of hydrophobic peptides containing a conserved motif, PR, was identified. Conclusion: Two bFGF mimic heptapeptides binding to FGFR were selected, which may be used as the candidates for bFGF agonist.

Key words： Phage display Heptapeptide Basic fibroblast growth factor Fibroblast growth factor receptor

Received: 28 December 2005 Published: 25 May 2006

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Cite this article:

. Selection of bFGF mimic peptide by phage display. China Biotechnology, 2006, 26(05): 7-10.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I05/7

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