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Abstract Objective To obtain for the high expression of Herpes Simplex Virus Type 1（HSV-1）Glycoprotein D gene. Methods The Herpes Simplex Virus Type 1（HSV-1）Glycoprotein D(gD1) gene fragment containing dominant antigen epitopes s confirmed by computer analysis was cloned by PCR technical and inserted into plasmid vector pTrxA, then the recombinant plasmid was transformed into Rosetta 。The expressed product was analyze by SDS-PAGE. Results 930 bp gene fragment was amplified by PCR as anticipated. Nucleotide sequencing showed a 100 % homology with that of the published sequence in GenBank. The molecular weight of the expressed protein was about Mr48000(Dalton) , Western blotting indicated that the antigenicity of the protein was good. Conclusion The plasmid pTrxA-gd1 was constructed and a high efficiency expression of the gd1 gene from Herpes Simplex Virus Type 1（HSV-1）strain was made. The expressed product shows a good antigenicity.
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Received: 24 October 2005
Published: 25 May 2006
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