Expressing  Purification and Identification of Neuritin Gene in the E.coil

China Biotechnology

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Expressing Purification and Identification of Neuritin Gene in the E.coil

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Abstract Neuritin is a new neurotrophic factor found rectently.In order to identify the function of Neuritin clearly . the coding sequence of human neuritin was amplified by PCR from neuritin cDNA ,this fragment digested by NOcI and NotI was inserted into pET32a by T4 ligase and transformed into E.coil BL21 then the recombinant plasmid named pET32a-neuritin was constructed successfμlly .neuritin was expressed distinctly after inducing by IPTG.The product was identified as neuritin by SDS-PAGE and Wester blot analysis .The expression production was purified on Ni2+-NTA column .

Key words： Purification human neuritin prokaryotic expression cloning

Received: 26 December 2005 Published: 25 April 2006

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. Expressing Purification and Identification of Neuritin Gene in the E.coil. China Biotechnology, 2006, 26(04): 46-50.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I04/46

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