Comparison of Cellular Immunity Raised by a Novel Adjuvant DC-Chol with Different HBsAg Preparations

China Biotechnology

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Comparison of Cellular Immunity Raised by a Novel Adjuvant DC-Chol with Different HBsAg Preparations

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Abstract Objective: To screen candidate antigen for therapeutic HBV vaccine with a novel adjuvant DC-Chol. Methods: BALB/c mice were injected with DC-Chol liposome and HBsAg prepared from CHO and Yeast respectively. One week later, IL-4, IL-2, IFN-γwere measured by ELISA or ELISPOT. Results: The levels of IL-2, IFN-γof HBsAg from Yeast with DC-Chol liposome were 20 and 119 times higher respectively than those of HBsAg from CHO with DC-Chol liposome. ELISPOT assay showed that the counts of spot-forming cells of IL-4 and IFN-γof HBsAg from Yeast with DC-Chol liposome were 2.8 and 46.3 times higher respectively than those of HBsAg from Yeast with Al(OH)3. Conclusion: HBsAg prepared from Yeast together with DC-Chol liposome may be an appropriate candidate for therapeutic HBV vaccine .

Key words： CHO Yeast IL-2 IFN-γ ELISPOT DC-Chol

Received: 10 February 2006 Published: 25 March 2006

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. Comparison of Cellular Immunity Raised by a Novel Adjuvant DC-Chol with Different HBsAg Preparations. China Biotechnology, 2006, 26(03): 22-25.

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https://manu60.magtech.com.cn/biotech/ OR https://manu60.magtech.com.cn/biotech/Y2006/V26/I03/22

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