| 研究报告 |
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| Expression of Key Enzymes in Glycerol Synthesis of Candida Glycerolgenesis in Saccharomyces Cerevisiae W303-1A |
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Abstract Glycerol is an ideal osmotolerant medium. Two genes of GPD and GPP encoding glycerol-3phosphate dehydrogenase and glycerol-3phosphate phosphatase, key enzymes in glycerol synthesis, were cloned from Candida glycerolgenesis WL2002－5 using PCR method. In Escherichia coli JM109, enough GPD and GPP genes were amplified using Tvector, furthermore, expression plasmids pYX212－GPD containing GPD gene and pYX212－GPP containing GPP gene were constructed, which were introduced into Saccharomyces cerevisiae W303－1A using LiAc transformation method successfully. Primary results showed that the biomass of pYX212－GPD/S. cerevisiae W303－1A was higher than those of pYX212－GPP/S. cerevisiae W303－1A and the wild type during the fermentation. After 72h fermentation, introduction of GPD gene could increase glycerol production to about 12mmol/L, while GPP gene had little effect on glycerol production (only 4mmol/L) in comparison with the wild type.
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Received: 25 January 2006
Published: 25 January 2006
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