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Abstract Objective: To research the activity of antibacterial proteins isolated from Ｍusca domestica during larvaepupae metamorphosis. Methods: The 5dayold larvae of Musca domestica were injured with a needle. 24h later, the larvae which had changed into pupae were selected. The antibacterial protein was isolated by a fourstep protocol including grinding, heating, CMsepharose F.F. cationexchange chromatography and another CMsepharose F.F. chromatography. Antibacterial activities of samples were tested by an ultrasensitive radial diffusion assay using Staphylococcus aureus as the indicator organism. The molecular weight of the isolated protein was determined by SDSPAGE. Results: The molecular weight of this isolated protein was 43kDa, and its antibacterial activity was strong. Conclusions: A kind of protein with strong antibacterial activity can be produced in the Musca domestica during larvaepupae metamorphosis.
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Received: 06 February 2006
Published: 25 January 2006
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